Gelatin Nanoparticles for Targeted Oligonucleotide Delivery to Kupffer Cells - Analytics, Formulation Development, Practical Application
نویسندگان
چکیده
Asymmetrical flow field-flow fractionation (AF4) offers unique separation properties for macromolecules, colloids, particles, and even cells from human or animal origin. At the same time the characterization of non viral colloidal drug delivery systems by means of AF4 is barely described in literature. Here, three different analytical tasks investigating gelatin nanoparticles as drug delivery system were executed utilizing AF4 coupled with multi-angle light scattering (MALS), UV, and refractive index (RI) detection respectively. In a comparative study, several batches of gelatin bulk material were characterized for their molecular weight distribution. Based on these data the exact requirements on gelatin for the realized development of a one-step desolvation process for gelatin nanoparticle synthesis could be described. Following the loading of surface-modified gelatin nanoparticles with a double-stranded oligonucleotide revealed systematical limits of the analytical approach. And finally, the quantification of the PEGylation of gelatin nanoparticles could successfully be completed. In sum, the presented results demonstrate the versatile applicability of asymmetrical flow field-flow fractionation also in the field of colloidal drug delivery systems.
منابع مشابه
Dissertation zur Erlangung des Doktorgrades der Fakultt fr Chemie und Pharmazie der Ludwig-Maximilians-Universitt Mnchen - NANOPARTICLES FOR RNA INTERFERENCE - NOVEL PRECLINICAL FORMULATIONS FOR SIRNA MEDIATED GENE THERAPY
1 1 GENERAL INTRODUCTION 3 1.1 Endothelial related diseases 3 1.2 siRNA and the principle of RNA interference 8 1.3 RNA interference in research and therapy 13 1.4 Viral and non-viral gene delivery 14 1.5 Nanoparticles: definitions, history and current state of knowledge 16 1.6 Gelatin based nanoparticles as colloidal drug delivery vectors 18 1.7 Body distribution of nanoparticles 19 1.8 Nanopa...
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